Informatics Publishing Limited

Rekha Rajeevkumar ¹, P. Rajeevkumar ², T. Vetrichelvan ³, D. Nagavalli ³

Affiliations
1 Department of Pharmaceutical Analysis, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka - 574 143, IN
2 Department of Pharmaceutics, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka-574143, IN
3 Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur, Kancheepuram, Tamilnadu-603319, IN

Abstract

A validated reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed for the estimation of moprolol in the pharmaceutical preparation using RPC₁₈ column. The mobile phase (Acetonitrile:Methanol:Water) was pumped at a flow rate of 1ml/min in the ratio of 15:50:35 and the eluents were monitored at 274nm at 30°C. The intra and interday precision was found to be less than 2% showing high precision of the assay method. The % recovery of the method was more than 99% and RSD did not exceed 2% indicating high degree of accuracy of the proposed HPLC method. The %RSD for the robustness testing was also less than 2%.The proposed HPLC method can be used for the estimation of moprolol in tablet dosage forms.

Keywords

Moprolol, RP-HPLC, Validation, Recovery, Tablet Dosage.

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P. Rajeevkumar ¹, N. Subramanian ², Rekha Rajeevkumar ³

Affiliations
1 Department of Pharmaceutics, Karuna College of Pharmacy, Thirumittacode, Palakkad - 679533, Kerala, IN
2 Department of Pharmaceutical Technology, Anna University of Technology, Tiruchirappalli - 24, T.N., IN
3 Department of Pharmaceutical Analysis, Karuna College of Pharmacy, Thirumittacode, Palakkad - 679533, Kerala, IN

Abstract

A simple, precise and accurate difference spectroscopic method has been developed for the estimation of lincomycin hydrochloride (LMH) in bulk and pharmaceutical dosage form. The proposed method is based on the measurement of absorbance of Lincomycin hydrochloride at maxima 209 nm and minima 220 nm. The measurement value is the amplitude of maxima and minima between two equimolar solutions of the analyte in different chemical forms, which exhibits different spectral characteristics. Since the drug was freely soluble in distilled water, a stock solution (1mg/ml) was prepared with distilled water. Further dilution was made by using 0.1N sodium hydroxide and phosphate buffer of pH6.8 separately. The maxima and minima in the difference spectra of lincomycin hydrochloride were at 209 nm and 220 nm, respectively. Difference in absorbance between these maxima and minima was calculated to find out the amplitude. This amplitude was plotted against concentrations. The drug obeyed the beer's law in the concentration range 5-25μg/ml and showed good correlation. The result of analysis was validated by recovery studies.

Keywords

Lincomycin Hydrochloride, Difference Spectroscopy, Pharmaceutical Dosage Forms.

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